Results of the harmful effects of water pollutants to green algae ( Scenedesmus subspicatus) in the cell multiplication inhibition test

Abstract

In the Scenedesmus cell multiplication inhibition test, 68 potentially hazardous substances were examined to determine the effect concentrations (EC). The tests were conducted in accordance with the test procedure DIN 38 412, Part 9 (draft standard). The green alga Scenedesmus subspicatus CHODAT was cultivated as the test organism. Twenty-five substances were examined according to the standardized test procedure. The procedure was modified somewhat for the examination of volatile and/or strongly smelling substances. For 21 of the tested substances the concentrations for the 72 h and/or 48 h E B C 10 values were in the concentration range 0.0001–2 mg 1 −1. Thus, they prove very/highly toxic to the cell multiplication of Scenedesmus subspicatus. When compared with the results of the 21d Daphnia reproduction test, monobromoacetic, monochloroacetic acid, chloroacetaldehyde and chloramine T proved very harmful to green algae.