Abstract
With a view to obtain the data on intraspecific diversity of Hantaviruses circulating in the territory of the Saratov Region, carried out has been molecular-genetic testing of the field and clinical material. Using PCR assay, Hantavirus RNA has been detected in 25 samples obtained from small mammals and 15 blood samples of HFRS patients. Sequencing of 14 hantavirus RNA-isolates from rodents and 3 RNA-isolates from clinical material has made it possible to identify their taxonomic appurtenance to Puumala virus. Phylogenetic analysis of RNA-isolates has revealed that all of them are characterized by high degree of homology in between themselves (99.8 %) and substantial distinction from the known Puumala strains, frequently occurring in Russia (3.6-21 %). Therewith, verified has been circulation of Puumala virus in the territory of the Saratov Region using molecular-genetic techniques. Identified genetic differences of Saratov RNA-isolates, in particular, opens a further prospect for allocation of the sites where people can contract HFRS infection.
Highlights
With a view to obtain the data on intraspecific diversity of Hantaviruses circulating in the territory of the Saratov Region, carried out has been molecular-genetic testing of the field and clinical material
Using PCR assay, Hantavirus RNA has been detected in 25 samples obtained from small mammals and 15 blood samples of HFRS patients
Sequencing of 14 hantavirus RNA-isolates from rodents and 3 RNA-isolates from clinical material has made it possible to identify their taxonomic appurtenance to Puumala virus
Summary
Эпизоотологическое обследование проводилось на территории зеленой зоны Саратова с определением общей численности мелких млекопитающих, а также с характеристикой состояния популяций основных носителей хантавирусов – возбудителей ГЛПС. Ретроспективный анализ литературных данных показал, что в 80–90-х годах прошлого столетия на этих территориях были зарегистрированы эпидемически активные природные очаги ГЛПС, отмечался высокий процент инфицированных грызунов, имели место массовые случаи заражения людей [1]. Зеленая зона Саратова, как наиболее часто посещаемая горожанами зона отдыха, может быть отнесена к территориям повышенной эпидемиологической опасности для людей. Лабораторные исследования суспензий органов мелких млекопитающих (смешанный пул легких и почек) на наличие антигенов и РНК хантавирусов проводили с использованием методов ИФА и ПЦР. Результаты выявления РНК хантавирусов в суспензиях органов мелких млекопитающих, отловленных в зеленой зоне Саратова
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
