Abstract
Currently, the detection and identification of viruses that cause respiratory and influenza-like illness (ILI) is one of the main tasks of public healthcare. In 2016-2017, nasopharyngeal swabs were collected from 200 sick children with ILI aged 0 to 5 years. Detection of pathogens in ILI patients was carried out by RT-LAMP amplification method. In 43 % of patients ILI viruses were detected. Among all confirmed cases of viral infection, a respiratory-syncytial virus was detected in 49 %, rhinoviruses in 16 %, coronaviruses in 9 %, parainfluenza viruses in 6 %, and human metapneumoviruses in 2 % of samples. Influenza A viruses were found in 6 % of infected specimens. The results obtained demonstrate the effectiveness of using RT-LAMP amplification in differential laboratory diagnostics of the ILI of viral etiology.
Highlights
In 2016-2017, nasopharyngeal swabs were collected from 200 sick children with influenza-like illness (ILI) aged 0 to 5 years
The results obtained demonstrate the effectiveness of using RT-Loop Mediated Isothermal Amplification (LAMP) amplification in differential laboratory diagnostics of the ILI of viral etiology
Brief reportsНакаучи Мина – PhD, старший научный сотрудник, Центр вирусологических исследований, Национальный институт инфекционных заболеваний (Toyama 1-23-1, Shinjuku-ku Tokyo 162-8640, Japan) Nakauchi Mina – PhD, Senior Research Officer, Virus Research Center, National Institute of Infectious Diseases (Toyama 1-23-1, Shinjuku-ku Tokyo 162-8640, Japan)
Summary
Khishigmunkh Ch. 1, Enkhsaykhan D. 1, Darmaa B. 1, Amarzhargal Y.A. 1, Nyamkhuu D. 1, Tsogtsaykhan S. 2, Nakauchi M. 3, Nymadawa P. 1. 1. Currently, the detection and identification of viruses that cause respiratory and influenza-like illness (ILI) is one of the main tasks of public healthcare. Detection of pathogens in ILI patients was carried out by RT-LAMP amplification method. For citation: Khishigmunkh Ch., Enkhsaykhan D., Darmaa B., Amarzhargal Y.A., Nyamkhuu D., Tsogtsaykhan S., Nakauchi M., Nymadawa P. Results of identification of infectious agents of influenza and other acute respiratory infections with the method of RT-LAMP. Главными возбудителями острых респираторных заболеваний являются вирусы гриппа А и В, а также другие вирусы, вызывающие гриппоподобные заболевания (ГПЗ). Для выявления возбудителей инфекционных заболеваний стали широко применять метод изотермальной ампликации Loop Mediated Isothermal Amplification (LAMP). Цель исследования состояла в выявлении некоторых вирусных возбудителей острых респираторных заболеваний с помощью метода RT-LAMP в носоглоточных смывах, взятых у детей младшего возраста
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