Result of embryo transferred research work in beef cattle and dairy cattle

Abstract

In the study, 6 donors (n=6) cow of Selenge (n=3) and Alatau breeds (n=3) were selected for superovulation. Superovulation treatment and artificial insemination (AI) of Donor Cow Regardless of the estrous cycle, Insert Controlled Internal Drug Release (CIDR; Hamilton, New Zealand) to Donor cow and from 5th day after inserting CIDR, diminishing inject Follicle Stimulating Hormone (FSH; Kyoritsu Seiyaku Corporation, Japan.) 36IU every 12 hours for 4 days. From the 3rd day after injecting FSH, inject Dinoprost Tromethamine (PGF2α; LutalyseTM, Upjohn, U.S.A.) 30mg in the morning and 15mg in the noon. From the 4th day after injecting FSH, remove the CIDR in the morning. The next day of removing the CIDR, inject Gonagorelin (GnRH; Dong-Bang, Korea.) 1000ug every 12 hours in the noon and did AI 4 times. Follicle Stimulating Hormone (FSH), Controlled Internal Drug Release (CIDR) and Prostaglandin (PG) were used for superovulation. Subsequently, Artificial Insemination (AI) was done for the donor cow and the embryo was collected after 7 and 8 days. Embryos were collected from donor cows of dairy cattle and beef cattle. Finally, good quality and fresh embryos (beef cattle’s 11 embryos, dairy cattle’s 12 embryos) were transferred to 23 recipients of Alatau and Selenge young cows in Mongolia. For synchronization treatment of recipient, cows injected 0.5ml estrogen, 1ml progesterone with CIDR implantation and injected 5.0 ml Lutalyse for 7 days, remove CIDR for 8 days and inject 2,5 ml of gonadotropin on the evening of the 9th day.