RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN CARROT

Abstract

Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Recently, molecular markers based on restriction fragment length polymorphism (RFLP) have been shown to be highly useful and efficient gene markers in other plant and animal species.We have used a total of 40 carrot strains (inbreds, varieties, species) for this study. Genomic DNA probes cloned in pGEM (Promega) plasmid of Escherichia coli were hybridized with genomic DNA of restriction enzymes. We have found RFLP variation to be extensive in Daucus, even among related cultivation genetic stocks. The implications of these results in the germplasm diversity in Daucus will be discussed.