Abstract
A human acute myelogenous leukemia cell line (KG-1) was used to study the humoral regulation of leukemic cell proliferation in diffusion chambers implanted in mice. The mice were treated with several agents known to enhance normal granulopoiesis. Leukemic cell proliferation was measured by total cell numbers and in vivo colony formation in plasma clot. Greatest leukemic cell growth was observed when the host mice were treated with cyclophosphamide. Administration of glucan or endotoxin stimulated growth to a significant but lesser degree. Pretreatment of the mice had no agreeable effect on leukemic cell maturation. To determine whether enhanced leukemic cell proliferation correlated with colony-stimulating activity (CSA) levels in the host animals, sera were collected daily and assayed in agar cultures using mouse bone marrow and KG-1 cells as targets. Sera from endotoxin-treated mice were found to be superior to sera from mice injected with cyclophosphamide in supporting in vitro colony formation. We conclude that there are humoral factor(s) other than CSA which are important in stimulating human leukemic cell growth in diffusion chambers in mice.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
