Responses to ocean acidification and diurnal temperature variation in a commercially farmed seaweed, Pyropia haitanensis (Rhodophyta)

Abstract

ABSTRACTTo investigate carbon and nitrogen metabolism in Pyropia haitanensis in response to the combined conditions of ocean acidification and diurnal temperature variation, maricultured thalli were tested in acidified culture under different temperature treatments. The results showed a combined effect of ocean acidification and diurnal temperature difference on the C and N metabolism and growth of P. haitanensis. In acidifed culture, algal growth, maximum photosynthetic rate, nitrate reductase (NR) activity, amino acid (AA) content and AA score (AAS) were more significantly enhanced in seaweed under diurnal temperature variation than in seaweed at constant temperature. In acidified seawater, soluble carbohydrates in P. haitanensis increased due to greater dissolved inorganic carbon (DIC), whereas soluble proteins decreased. Under the diurnal temperature treatment, higher temperature during the light period enhanced accumulation of algal photosynthates, whereas lower temperature in the dark period reduced energy consumption, resulting in enhanced algal growth, AA content and AAS. We concluded that suitable diurnal temperature difference would be conducive to C fixation and N assimilation under ocean acidification. However, excessively high temperatures would depress algal photosynthesis and increase energy consumption, thereby exerting a negative effect on algal growth.