Abstract
Lecithin in mixed micelles attenuates cytotoxicity by interacting with proteins or other cell inclusions. The reaction was then captured via magnetic beads binding approach. This research proposal was designed to discover the impacts of lecithin on apoptosis relevant factors (including caspase 3/8/9 and PARP) by Western Blot and specific protein binding mechanism of lecithin detoxification pathway, using caco-2 cell model. Preliminary investigation of the properties of the proteins was conducted by MS (Mass Spectrometry) couplet to AC (Affinity Chromatography) assay. Further research of particular proteins was to identify the size and peptide sequence. This study also provided solid evidence for the previous research result, which was lecithin reduce cytotoxicity induced by SDC contained mixed micelles.
Highlights
It is believed that if Lecithin exists in mixed micelles, it has the ability to attenuate the cytotoxicity of bile salts that is one of the ingredients in the mixed micelles, which can be widely used as important drug delivery systems by enhancing the permeability and solubility of some insoluble oral drugs across gastrointestinal epithelia[1][2]
SDC (Sodium Deoxycholate), known as bile salts, has cytotoxicity when utilized as an essential role in drug carriers, while Lecithin in contrast can reduce a certain degree of this toxicity
After 3-day of cell growth, the cells were treated with same volume of Lecithin/SDC mixed micelles and physiological saline in replace of culture medium and 2 hours later, 20μL of distinct concentration of Lecithin was added to every plate
Summary
It is believed that if Lecithin exists in mixed micelles, it has the ability to attenuate the cytotoxicity of bile salts that is one of the ingredients in the mixed micelles, which can be widely used as important drug delivery systems by enhancing the permeability and solubility of some insoluble oral drugs across gastrointestinal epithelia[1][2]. Optimum use of lecithin in the Lecithin/SDC mixed micelles is rather significant to attenuate the cytotoxicity. When it comes to real applications as oral drug carriers, the molecular mechanisms of how Lecithin reducing the cytotoxicity need to be considered and investigated. In this study, we designed a series of experiments to evaluate the of impact of lecithin on molecular level and experimental verifications of what Lecithin specific binding protein is. It was integrated with the former cellular level results to better make clear of protein level interaction
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