Responses ofLotus corniculatus to environmental change. 2. Effect of elevated CO2, temperature and drought on tissue digestion in relation to condensed tannin and carbohydrate accumulation

Abstract

Clonal plants of three genotypes of Lotus corniculatus (cv Leo) were grown in eight controlled environments under combinations of two temperature regimes, two CO2 concentrations and two watering regimes. Condensed tannins (proanthocyanidins), in-vitro digestibility, initial rates of gas evolution (as an indicator of the initial rates of fermentation of the substrate), volatile fatty acid evolution, and non-structural carbohydrate (NSC) levels were determined in leaves, stems and roots at full flowering. Under control conditions (average midsummer conditions in the United Kingdom) the total condensed tannin content of leaves varied six-fold between genotypes but condensed tannin contents in stems and roots were similar. Condensed tannin levels were significantly increased in leaves and stems of all three genotypes by doubling the CO2 concentration while raising the temperature towards the optimum for growth significantly reduced condensed tannin levels. Drought stress significantly reduced condensed tannin levels in leaves and, particularly, in roots. Nutritive value was inversely related to condensed tannin levels in leaves and a negative relationship was observed between condensed tannin concentrations of more than 25–30 g kg−1 dry matter and the initial rates of gas evolution when subjected to in-vitro fermentation with rumen micro-organisms. In leaves, digestibility was significantly increased by drought and by increasing temperature but reduced by high CO2. In stems, digestibility was significantly increased by drought, but not significantly affected by increasing temperature, or by high CO2 alone. In roots, digestibility was significantly increased by drought, and decreased by increasing temperature or CO2. Increasing the growth temperature towards optimum growth reduced the content of NSC in all tissues with the greatest changes occurring in root tissue. Doubling the CO2 concentration increased NSC levels in leaves and stems with starch content more than doubled under high CO2 while, in roots, increased levels were only observed in combination with drought stress. There was a linear correlation between condensed tannin concentration and total NSC that was positive for leaves, neutral for stems and negative for roots. The relationship between carbohydrate levels and rates of gas production was negative for leaves and positive for stem and roots. © 1999 Society of Chemical Industry