Abstract
Weyers, J. D. B. and Paterson, N. W. 1987. Responses of Commelina communis stomata in vitro.— J. exp. Bot. 38: 631–641. Analysis of the kinetics of movements of Commelina communis L. stomata in vitro revealed a sequence of opening and closing phases dependent on the incubation medium used and the physiological state of the plant material. In buffer containing 50 mol m −3 KC1 the sequence of aperture changes appeared to be influenced by equilibration of cell water potentials with that of the medium and by solute fluxes (dependent and independent on metabolic activity). The results indicate that the stomatal aperture after several hours of incubation may not always provide a reliable quantitative estimate of the ability of the stomata to operate. As a consequence, modifications are suggested to the ways in which experiments using epidermal strips are carried out and reported.
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