Responses of Vegetation, Soil, and Microbes and Carbon and Nitrogen Pools to Semiarid Grassland Land-Use Patterns in Duolun, Inner Mongolia, China

Abstract

Previous studies have observed that increased precipitation positively affects primary production in semiarid grasslands in Inner Mongolia, while soil carbon (C) and nitrogen (N) strongly influence how ecosystems respond to precipitation as well as anthropogenic disturbances under different management strategies. Therefore, in this study, we investigated the storage of organic C and N in four grassland sites with similar flora and landforms but with different grazing intensities to characterize how the storage and concentrations of C and N respond to relief from grazing pressure and precipitation. The concentrations of soil organic carbon (SOC), soil total nitrogen (STN), microbial biomass carbon (MBC), and microbial biomass nitrogen (MBN), as well as the contents of soil bacteria and fungi in the 0–50 cm soil layers, were measured. The results showed that SOC, STN, MBC, and MBN storage varied greatly among the four grassland sites (p < 0.05), with all decreasing significantly with grassland degradation and increasing greatly with the exclusion of grazing, i.e., the establishment of natural grassland (NG). More than 90% of C and 95% of N stored in the soil were lost due to heavy grazing, but the losses were smaller in other nutrient pools (including the aboveground biomass, litter, and roots). Interestingly, the proportion of the particle size fractions (clay, silt, and sand) had a stronger effect on limiting the soil and microbial nutrient pools compared to precipitation. The limited range of C and N storage found in these grassland soils indicated that enclosed fencing was a valuable management tool with a high potential to sequester C in the top meter of the soil, showing a stronger effect than precipitation. This study provides a theoretical basis for improving grassland recovery in semiarid areas that have been heavily grazed.