Responses of two rice cultivars differing in seedling-stage nitrogen use efficiency to growth under low-nitrogen conditions

Abstract

Demand for low-input nitrogen sustainable rice is increasing to meet the need for environmentally friendly agriculture and thus development of rice with high nitrogen use efficiency (NUE) is a major objective. Hence, understanding how rice responds to growth under low-nitrogen conditions is essential to devise new ways of manipulating genes to improve rice NUE. In this study, using two rice varieties with different seedling-stage NUE obtained from previous field experiments, we investigated the physiological and molecular responses of young rice to low-nitrogen conditions. Our results suggest that glutamine synthetase (GS) and NADH-dependent glutamate synthase (NADH-GOGAT) play important roles in N assimilation of seedling rice roots under low-nitrogen conditions; the regulatory mechanisms of GS and NADH-GOGAT in seedling rice roots do not occur at the transcription level, and may be posttranscriptional; OsAMT1;1 play important roles in rice N acquisition by partially regulating N uptake under low-nitrogen conditions; and OsAMT1;1 and OsNRT2;1 also play important roles in rice N acquisition by partially regulating root growth and development under low-nitrogen conditions. The challenge for future studies is to characterize the functional roles of GS, NADH-GOGAT, OsAMT1;1, and OsNRT2;1 in young rice NUE using RNAi and mutant techniques.