Abstract
The present study was conducted with growing rats to examine effects of addition of 0.3, 1.0, 2.5, and 5% L-cystine to 10 or 25% casein diet on tissue ascorbic acid and on serum cholesterol, alpha-tocopherol, and ceruloplasmin activity. Addition of 0.3-1% cystine to 10% casein diet caused the maximum growth and addition of 5% cystine to 25% casein diet depressed the growth. Increases in liver levels of ascorbic acid and in serum levels of cholesterol and alpha-tocopherol were observed with addition of 5% cystine to 10 and 25% casein diets as compared to the diets without addition of cystine. Addition of 0.3-5% cystine to 10% casein diet and addition of 5% cystine to 25% casein diet caused a decreased activity of serum ceruloplasmin. The changes in liver ascorbic acid, serum cholesterol, and serum alpha-tocopherol and in ceruloplasmin activity by dietary cystine correlated with the changes in liver levels of non-protein sulfhydryl.
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