Responses of salicylic acid encapsulation on growth, photosynthetic attributes and ROS scavenging system in Lactuca sativa exposed to polycyclic aromatic hydrocarbon pollution

Abstract

Salicylic acid (SA) is a phytohormone that plays a key role in the regulation of the defense response against environmental variables in plants, and it provides increased yield and stress tolerance when exogenously applied to plants as a growth regulator. The role of SA-mediated signals in abiotic stress tolerance varies according to the species, stressor, application method, and dose. This study investigated the effects of salicylic acid (SA, 0.1 mg ml−1) or β-cyclodextrin encapsulated salicylic acid (e-SA, 0.1 mg ml−1) treatments on growth parameters, gas exchange, photosynthesis efficiency, and antioxidant capacity in lettuce seedlings exposed to polycyclic aromatic hydrocarbon pollution. Fluorene (FLN, 100 mg L−1) contamination resulted in a 27% growth rate and a 14% water content reduction in lettuce leaves. Significant suppressions of stomatal conductance, carbon assimilation, and PSII photochemistry were detected in plants under stress. FLN + SA and FLN + e-SA treatments regulated plant-water relations by stimulating proline accumulation and relieving stomatal limitations. As indicated by the high Fv/Fm ratio, photosynthesis efficiency was recovered in FLN + SA and FLN + e-SA group plants. FLN stress caused high oxidative stress in lettuce leaves and increased lipid peroxidation level by 40%. However, especially e-SA application to plants under stress, increased SOD activity by 3-fold and CAT activity by 80% and was successful in preventing H2O2 accumulation and lipid peroxidation. Both SA and e-SA treatments partially activated the AsA-GSH cycle. As a result, direct SA application was effective in mitigating stress-induced physiological limitations with high SA accumulation in the tissues, while encapsulated SA treatment was more effective in regulating photosynthetic and biochemical reactions, alleviating oxidative damage by activating the antioxidant defense, and promoting growth under stress with moderate SA accumulation.