Abstract
During metastasis tumor cells may be affected by a variety of environmental factors including intercellular substances and growth factors of host origin. We have studied this relationship on several cloned cell lines derived from a rat rhabdomyosarcoma selected on the basis of their metastatic potential. We show that the parental cell line (9–4/0) was stimulated to proliferate in vitro by Epidermal Growth Factor (EGF), pituitary Fibroblast Growth Factor (pFGF) and Eye Derived Growth Factor (EDGF) in the presence of 10% fetal calf serum (FCS). Six sublines which differed in their doubling time and metastatic potential were studied. These growth factors stimulate all these cells to some extent but some cell lines were more responsive to EGF than to EDGF or to FGF, while other cell lines were more responsive to EDGF and very little to EGF. One cloned cell line (14) was chosen to study the different parameters playing a role in growth factor stimulation. This growth factor-increased proliferation was independent on the FCS concentration and on the initial cell density.
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