Responses of grassland soil nematodes and protozoa to multi-year and single-year applications of dairy manure slurry and fertilizer

Abstract

In order to improve understanding of how long-term use of manure affects nitrogen cycling processes, the effects of multiple years of manure applications on abundance of protozoa and nematode community structure were assessed. Plots of a grass sward in the Fraser Valley of British Columbia were either left untreated or were treated with dairy manure slurry or fertilizer, each at 50 or 100 kg NH 4–N ha −1, two to four times per year for six consecutive years. Nematode community structure and protozoan abundance were determined at 19 sample dates during the fourth (1997), fifth (1998) and sixth (1999) years of application. Protozoa, bacterivorous nematodes and fungivorous nematodes were consistently more abundant in soil treated with manure at both rates than in fertilized and untreated soil, indicating that microbial turnover and flux of nutrients through the soil food web was enhanced in manured soil relative to fertilized or untreated soil. The Maturity Index (MI) and the MI2-5 were both reduced by fertilization and manure, relative to the control. The MI for the manure treatment was lower than for the fertilizer treatment as a result of greater relative abundance of enrichment opportunist nematodes in manure-treated soil. Accordingly, the MI2-5 did not differ between the manure and fertilizer treatments, suggesting that with the exception of enrichment opportunists fertilizer and manure have similar effects on structural complexity of the soil food web. Populations of micro-fauna were also assessed through 1998 and 1999 in subplots that had been treated with manure or fertilizer for four years and stopped receiving manure or fertilizer in 1998, and in subplots given manure in 1998 that had previously either been fertilized or left untreated. Protozoa and bacterivorous and fungivorous nematodes remained more abundant through 1998 and 1999 in previously manure-treated plots than in previously fertilized plots, indicating that the cumulative effects of manure application on enhancement of microbial production can be detected through at least two growing seasons after applications cease. Application of manure for one year to previously non-treated or fertilized soil raised the abundance of protozoa and bacterivorous and fungivorous nematodes to levels comparable to continuously manured soil.