Responses of glucosensing system to glucose in Japanese flounder Paralichthys olivaceus fed diets with different carbohydrate content.

Abstract

A 10-week feeding trial was conducted to investigate the response of glucosensing system to glucose in Japanese flounder Paralichthys olivaceus (initial body weight: 7.14 ± 0.10 g) fed diets with different carbohydrate content. Two experimental diets were designed as carbohydrate free (CF) and suitable carbohydrate (SC) supplementation, respectively. The dietary carbohydrate contents were 0.93% and 15.6%, respectively. After a 10-week feeding trial, a glucose tolerance test (GTT) was performed. Results showed that after the last meal in the feeding trial, the blood glucose of fish fed with diet CF peaked at 3 h (4.64 ± 0.29 mM), the duration of hyperglycemia was about 5 h (1-6 h). The blood glucose in SC group peaked at 9 h (3.28 ± 0.66 mM), and the duration of hyperglycemia was approximately 6 h (6-12 h). After GTT, blood glucose reached the first peak at 6 h both in the two groups, and the duration of hyperglycemia was obvious 24 h. During the 3-12 h after injection, blood glucose level in SC group was significantly higher than that in CF group. However, blood glucose level in group SC was significantly lower than that in group CF at 24 h. The blood glucose level decreased to half of the peak at 10.97 h after injection of glucose in SC group and at 27.26 h in CF group. The 6-24 h clearance ability in SC group (6.57 ± 1.68%/h) was significantly higher than that in CF group (2.81 ± 1.11%/h). Compared with CF diet, SC diet significantly increase the expression of glucosensing-related genes including glucose facilitative transporter type 2, glucokinase, inward rectifier K+ channel pore type 6.2, sulfonylurea receptor, carnitine palmitoyltransferase 1b, hydroxyacyl-CoA dehydrogenase, cytochrome c oxidase subunit 4, mitochondrial uncoupling protein 2a, liver X receptor, sodium/glucose co-transporter 1, a heterodimer of type 1 receptor subunits depending on T1R2 + T1R3 in liver and intestine. Meanwhile, activities of glucokinase, pyruvate kinase and glycogen synthase in liver, and hepatic glycogen content were also increased. In conclusion, glucosensing systems in Japanese flounder are responsive to dietary carbohydrate levels, especially the suitable dietary carbohydrate level, at which the glucose tolerance capacity of Japanese flounder was improved.