Responses of differentially expressed proteins and endogenous hormones in winter rapeseed (Brassica rapa L.) roots under water deficit stress

Abstract

AbstractWinter rapeseed (Brassica rapa L.) can well‐adapt to environmental conditions such as barrenness, water deficit and low temperature in arid and semi‐arid planting regions and is the preferred rapeseed type. In this study, we analysed changes of root system morphology, antioxidant enzyme activity, endogenous hormone contents and differentially expressed proteins (DEPs) under control (CK), slight water deficit (SWD, 50–55% of maximum field water capacity), moderate water deficit (MWD, 40–45% of maximum field water capacity) and high water deficit (HWD, 30–35% of maximum field water capacity) conditions. Winter rapeseed experienced taproot elongation, decreased taproot diameter and increased lateral root number, under water deficit stress. The accumulation of reactive oxygen species (ROS) can cause membrane system peroxidation, and antioxidant enzyme activity increases to remove ROS. Changes in jasmonic acid (JA), salicylic acid (SA), cytokinin (CTK), auxin (IAA) and gibberellin (GA) levels promote the absorption of water and minerals by driving changes in the root system architecture to resist water deficit stress. A proteomic analysis has shown that DEPs are involved in energy metabolism, antioxidation response, osmotic regulation, hormone signal transduction, protein metabolism and the stress response, and these proteins are located in the peroxisome, chloroplast, mitochondrion, cell wall, vacuole, cytoplasm, extracellular space and cell membrane. In this study, multiple DEPs (malate dehydrogenase cytoplasmic 1 OS, 14‐3‐3‐like protein GF14 Psi, GA 3‐beta‐dioxygenase, glutathione reductase and jasmonate‐inducible protein) were involved in the root system architecture, revealing the complexity of the root response to water deficit. Significant/extremely significant synergistic relationships were observed between antioxidant enzyme activity and endogenous hormone contents. In conclusion, ROS, endogenous hormones and stress‐related proteins work synergistically to control the root system architecture of winter rapeseed roots in response to water deficit stress.