Response to the Views and Opinions of Maningat, Seib, and Bassi Regarding McCleary et al (2013)

Abstract

ABSTRACTPhosphate cross‐linked starch, referred to as resistant starch 4 (RS4) is hydrolyzed much more rapidly under physiological conditions (i.e., pH 6, 37°C, pancreatic α‐amylase) than under the conditions used in AOAC dietary fiber method 985.29/AACC International Approved Method 32‐45.01 (the Prosky method), in which samples are incubated with thermostable bacterial α‐amylase at 95–100°C. For this reason, the author concludes that the Prosky method overestimates the dietary fiber content of these materials. A more accurate estimate of dietary fiber content is obtained with AOAC Method 2009.01/AACCI Approved Method 32‐45.01.