Response to oxidative stress generation in Rhodotorula glutinis and Candida tropicalis by thallium dithiocarbamate complexes

Abstract

Sodium diethyldithiocarbamate trihydrate (NaEt2dtc·3H2O) and sodium piperidine dithiocarbamate monohydrate (NaPidtc·H2O) reacted with thallium(I) carbonate to produce the complexes [Tl2(Et2dtc)2] C1 and [Tl2(Pidtc)2] C2. X-ray crystallographic analysis of the complexes elucidated their triclinic P-1 and monoclinic P21/n space groups, respectively, in addition to chelation via SS atoms from the uninegative ligands. Further, this analysis revealed the complexes' assembly as one-dimensional polymers of dimeric building blocks. The complexes (50 µg/ml) caused greater growth inhibitions in two pathogenic yeasts comparing with the ligand salts and cycloheximide. The ligand salts, respectively, inhibited R. glutinis with 15.02 and 15.14 % and C. tropicalis with 11.09 and 11.45 %, while cycloheximide, C1 and C2 offered inhibitions with 26.16, 38.9 and 46.4 % in R. glutinis and 13.4, 29.8 and 38.9 % in C. tropicalis, respectively. Further, the thallium complexes significantly affected the yeasts' soluble proteins {cycloheximide, C1 and C2 afforded 7.06 ± 0.05, 6.11 ± 0.08 and 5.43 ± 0.05 mg/g fresh weight (for R. glutinis) and 7.67 ± 0.15, 7.26 ± 0.06 and 6.025 ± 0.14 mg/g fresh weight (for C. tropicalis)} and total antioxidants {cycloheximide, C1 and C2 afforded 10.15 ± 0.21, 11.27 ± 0.16 and 14.97 ± 1.3 mg/g protein (for R. glutinis) and 19.4 ± 0.65, 16.0 ± 0.24 and 17.19 ± 0.55 mg/g protein (for C. tropicalis)} in addition to the catalase and peroxidase enzyme activities.