Abstract
In a recent contribution to this journal Grisedale and Van Daal concluded that a single STR analysis of all available template DNA is to be preferred over replicate analyses and a consensus approach when analyzing low template DNA samples. A single STR analysis approach does not allow for an assessment of the validity of the resulting DNA profile. We argue that the use of replicate amplifications is the best way to objectively quantify the extent of the stochastic variation in the data. By applying consensus methodology and/or a probabilistic model, the interpretation of the data will therefore be more objective and reliable.
Highlights
In a recent contribution to this journal Grisedale and Van Daal concluded that a single short tandem repeat (STR) analysis of all available template DNA is to be preferred over replicate analyses and a consensus approach when analyzing low template DNA samples
Statistical models that incorporate the probabilities of stochastic effects to provide an evidential value to DNA profile comparisons have previously been described [1,2]
The optimal way to apply this model and the use for mock case samples were extensively studied by Benschop and colleagues [5,6,7]. In their recent contribution to Investigative Genetics, Grisedale and Van Daal report on the comparison of STR profiling from low template DNA (LT-DNA) extracts with and without the consensus profiling method [8]. Based on their studies they conclude that a single STR analysis of all available template DNA is to be preferred over replicate analyses and a consensus approach
Summary
In a recent contribution to this journal Grisedale and Van Daal concluded that a single STR analysis of all available template DNA is to be preferred over replicate analyses and a consensus approach when analyzing low template DNA samples. The consecutive interpretation of these DNA profiles can be very complex due to stochastic effects. One of the major challenges when interpreting LT-DNA results is to distinguish artifact peaks from actual alleles derived from DNA present in the sample.
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