Response to Bombyx mori nucleopolyhedrovirus infection in silkworm: Gut metabolites and microbiota

Abstract

The diversity of microbiota and metabolites in the digestive tract gut is important in physiology and homeostasis, nutrient uptake and virus infection. In lepidopteran insect model silkworms, little is known about how microbiota and metabolites are altered after oral infection with BmNPV. Herein, we used 16S rDNA sequencing and metabolomics to show that the gut microbiota and metabolites of silkworm midgut are significantly altered after BmNPV infection. Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment of flavone and flavonol biosynthesis, glycosyltransferases, and electron transfer carriers signaling pathways, suggesting potential roles in viral infection. Infection also changed the abundance of metabolites in the digestive tract gut, where most pathways were related to metabolism of amino acids, fatty acids and other pathways (e.g., citrate cycle). In addition, a correlation was observed between digestive tract gut microbiota and metabolites. These results shed light on the interaction between digestive tract gut microbiota, metabolites and host-virus interaction, and enhance our understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm.