Response Surface Optimization of Ultrasound Assisted Extraction (UAE) of Phycocyanin from Microalgae Spirulina platensis

Hady Hadiyanto,Suttrisnorhadi Suttrisnorhadi

doi:10.9755/ejfa.2015-05-193

Hady Hadiyanto, Suttrisnorhadi Suttrisnorhadi

Open Access

https://doi.org/10.9755/ejfa.2015-05-193

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Abstract

Phycocyanin extracted from Spirulina platenis has been linked to many food colorant and health applications. The extraction of this compound by using conventional method resulted low yield and longer processing time. This research was aimed to determine optimum process conditions of ultrasound assisted extraction (UAE) of phycocyanin compound from microalgae Spirulina platensis. The response surface methodology (RSM) was employed to search UAE optimum conditions among set of variables: temperature (T=30-60oC), time (20-50 min) and frequency (28-42 Hz). The result showed that under unoptimized conditions, the yield of phycocyanin extracted by conventional method was 11.13 % while ultrasound could increase the yield up to 13.61 %. The optimization by using RSM showed that the maximum yield was achieved at 15.7 % and EC50 of 85.78 g/mL at temperature of 52.5oC, extraction time of 42 min and ultrasound frequency of 42 Hz. The conclusion of this research was ultrasound could improve significantly the efficiency of extraction of phycocyanin from microalgae and the obtained optimal conditions can be potentially scaled up to isolate phycocyanin for large production in the food and pharmaceutical industries.

Highlights

Sustainable production of C-Phycoyanin (CPC) from algae Spirulina platensis has been intensively studied due to their outstanding benefits
Various novel extraction methods have been introduced for the extraction of active components from plants, such as ultrasonic-assisted extraction (UAE), supercritical fluid extraction and enzymatic extraction (Hardlei et al, 2007; Yang et al, 2009; Vinatoru, 2001; Sivansankari et al, 2014)
This research was aimed to investigate the effect of ultrasound assisted extraction (UAE) variables: temperature, time and frequencies in extraction of phycocyanin from microalgae Spirulina sp and to employee Response Surface Methodology (RSM) as a useful engineering tool to optimize process conditions in order to achieve high yield and high antioxidant activity

Summary

Introduction

Sustainable production of C-Phycoyanin (CPC) from algae Spirulina platensis has been intensively studied due to their outstanding benefits. Various novel extraction methods have been introduced for the extraction of active components from plants, such as ultrasonic-assisted extraction (UAE), supercritical fluid extraction and enzymatic extraction (Hardlei et al, 2007; Yang et al, 2009; Vinatoru, 2001; Sivansankari et al, 2014). Among these methods, the UAE is an efficient extraction technique due to shorter extraction time stimulated by its acoustic cavitation power. The ultrasound assisted extraction (UAE) is recently considered as an efficient technique to extract low molecular weight

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