Abstract
Mimosa pudica Linn is a well-known perennial herb and is traditionally used in ayurvedic medicine for the treatment of various illnesses. Despite its abundance in nature, the therapeutic potential of this invasive weed is deemed to be underappreciated in Malaysia. Previous studies have found an abundance of bioactive compounds associated with potent antioxidant properties in all parts of the plant. However, the optimum parameters required for the extraction of antioxidant compounds are still unknown. Therefore, the present study aimed to optimize the solvent extraction parameters of M. pudica using response surface methodology to enrich the accumulation of antioxidant compounds in the extracts. The effects of the optimized M. pudica extracts were then evaluated on the cell viability and glucose uptake ability in a 3T3-L1 adipocyte cell line. The highest total phenolic (91.98 mg of gallic acid equivalent per g of the dry extract) and total flavonoid content (606.31 mg of quercetin equivalent per g of the dry extract) were recorded when using 100% ethanol that was five-fold and three-fold higher, respectively, as compared to using 50% ethanol. The extract concentration required to achieve 50% of antioxidant activity (IC50 value) was 42.0 µg/mL using 100% ethanol as compared to 975.03 µg/mL using 50% ethanol. The results indicated that the use of 100% ethanol solvent had the greatest impact on the accumulation of antioxidant compounds in the extract (p < 0.05). Cell viability assay revealed that all extract concentration treatments recorded a viability level of above 50%. Glucose uptake assay using 2-NBDG analog showed that the cells treated with 50 µg/mL extract combined with insulin were five-fold higher than the control group. Given the high antioxidant and antidiabetic properties of this plant, M. pudica can be easily highlighted as a plant subject of interest, which warrants further investigation for nutraceutical prospects.
Highlights
IntroductionIs a perennial herb species that belongs to the family of Fabaceae
Mimosa pudica Linn. is a perennial herb species that belongs to the family of Fabaceae.It is commonly known as sensitive plant or shy plant and recognized for its shrinking reaction to contact as a defense mechanism
These findings suggest that the optimized M. pudica ethanolic extracts caused no harm to 3T3-L1 cells even at relatively high concentrations
Summary
Is a perennial herb species that belongs to the family of Fabaceae. It is commonly known as sensitive plant or shy plant and recognized for its shrinking reaction to contact as a defense mechanism. Diseases, such as diabetes, fever, wounds, ulcers, and dyspepsia [1]. This creeping perennial shrub is originated from tropical climate regions like Southeast Asia and can be found on roadsides, walkways, croplands, and home gardens in countries like. This plant species is dismissed as weeds owing to its invasive behavior, which causes inconveniences in home gardens and cropland maintenance
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