Abstract
Textile wastewater (TWW) causes many environmental problems, and colored substances must be removed from it before their discharge into the environment. An optimization process was analyzed using custom response surface methodology (RSM). In the present study, the effect of different combinations of eight strains was studied using equilateral triangle diagram (Minitab 14.0) and mixture experimental design to assess color and chemical oxygen demand (COD) removal during the growth of species. Under optimal conditions, the bacterial consortium developed was able to decolorize completely (>84%) the dye within 72 h. The color and COD removal were 89.97 and 84.71%, respectively. A significant increase in azoreductase, lignin peroxidase and laccase activities in the cells were obtained after complete decolorization. Ultraviolet (UV)–vis and nuclear magnetic resonance (NMR) spectroscopy analysis confirmed the biodegradability of the TWW by the developed bacterial consortium.
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