Response of wheat cells to ferrihydrite nanoparticles under model system in vitro

Abstract

High biological activity of nanoparticles (NPs) makes them promising for using in fertilizers composition. Design of such products is impossible without detection of active substance influence mechanisms on growth and development of plant as well as without detection of critical points, when influence of the investigated agent is at its maximum. Biogenic ferrihydrites (FH) are promising regulators of plant cell antioxidant system activity, also under edaphic stresses. Callus cultures are well known as convenient model system for screening of tissue and cell response to different environmental exposure. Investigation results of FH NP`s, which were produced by authors, influence on growth and development of callus culture of soft spring wheat mature wheat embryos of are presented in present article. The absence of competitive in relation to 2,4-D influence of involved levels of FH NPs (1 and 10 mg/l) on dedifferentiation process of embryo tissues was indicated. The presence of investigated agent induced the decrease of culture growth rate. However, postponed positive effect manifested in reduction of ageing rate after passing to fresh medium, the bigger, the higher had been FH NP`s level at the previous cultivation stage. The present effect may be of critical importance under field conditions under the influence of unfavorable edaphic and climatic factors, which adds further credence the potential of presented NPs applicability in agriculture.