Abstract
Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus A¢â‚¬â€œIndonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto , PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra , PSPT 9, Marta , and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia . Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato
Highlights
Whitefly-transmitted geminiviruses (WTGs) (Geminiviridae, Begomovirus) are known world wide as economically important pathogens causing serious diseases in food crops such as beans, peppers, cucurbits, cassavas, and tomatoes
We reported in this paper the application of non radioactive-labeled DNA probe for screening tomato genotypes for resistance to begomovirus
Visual symptoms developed following inoculation of three isolates of begomovirus was varied among different tomato genotypes (Table 2)
Summary
Whitefly-transmitted geminiviruses (WTGs) (Geminiviridae, Begomovirus) are known world wide as economically important pathogens causing serious diseases in food crops such as beans, peppers, cucurbits, cassavas, and tomatoes. Different isolates of tomatoinfecting begomoviruses were identified from different tomato growing areas at West Java, East Java, Central Java, and Yogyakarta (Kon et al 2003; Sudiono et al 2004; Aidawati et al 2005; Sukamto et al 2005; Tsai et al 2006). The use of insecticides has been proven effective only when the whitefly population is considered low. Molecular approach using spesific DNA probe for nucleic acid hybridisation was known as a promising method for detection of begomovirus infection in different host plants (Gilbertson et al 1991; Rom et al 1993; Lapidot et al 1997; Pico et al 1996; Rubio et al 2001).
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