Response of two barley cultivars to increasing concentrations of cadmium or chromium in soil during the growing period.

Abstract

The heavy metal contamination of soils is a serious environmental issue because excessive metal concentrations pose risks to the health of humans, animals, and plants. For this reason, the interest in understanding the toxic effects of metals on crop growth and physiology has increased in the last decades. A pot trial was performed in a greenhouse to evaluate the effects of contaminated soil with different concentrations of cadmium (Cd) or chromium (Cr) on barley growth and development. Two cultivars of barley were studied, Pedrezuela and CB502. Growth, chlorophyll content, chlorophyll fluorescence, and relative water content (RWC) were analyzed during the plant-growing period. After harvesting, the Cd and Cr contents in plant were analyzed. No significant differences were observed for chlorophyll content and chlorophyll fluorescence between control plants and those treated with Cd. In the case of Cr, a significant decrease of plant growth, chlorophyll content, chlorophyll fluorescence, and RWC was detected with respect to the control. The tolerance index (TI) and translocation factor (TF) were calculated. Data indicated that both varieties are tolerant to these metals; CB502 showed higher tolerance to Cr and Pedrezuela to Cd. The effect of Cd or Cr addition on nutrient concentrations in plants varied among elements and organs of the plant analyzed. The correlations between the physiological and agronomic studied traits were significant (p < 0.01, p < 0.001), so the stress induced by these metals affected the physiology and water relations of the plant, which provoked a decrease of plant biomass, especially in the plants treated with Cr.