Response of tissue phospholipid fatty acid composition to dietary (n-6) and replacement with marine (n-3) and saturated fatty acids in the rat

Abstract

Nine groups of weanling male rats were maintained for ten weeks on a fat-free semi-synthetic diet supplemented with 10% by weight of oil, composed wholly of gamma-linolenic acid-rich evening primrose oil, or replaced partly or completely (25%, 50%, 75%, and 100%) by marine or hydrogenated coconut oils. Plasma phospholipid content and phospholipid fatty acid composition of plasma, red blood cells, liver, kidney, and heart were determined. Replacement of marine oil as 2.5% of the diet caused a decrease of plasma phospholipid concentration to 60%, with no furthur decrease at higher proportions. Except in the heart, marine oil in combination with evening primrose oil caused an increase in 20:3(n-6) concentration, and a decrease in 20:4(n-6) levels in all tissues examined. The ratio 20:3(n-6)/20:4(n-6) increased with increasing marine oil supplementation to the diet, but not when marine oil was the sole source of dietary fatty acids (10%). Replacement of hydrogenated coconut oil did not affect 20:3(n-6) concentrations. A decrease in 20:4(n-6) was observed when hydrogenated coconut oil was supplemented to the diet at 10%, causing a state of EFA deficiency as shown by the elevation of 20:3(n-9). There was an increase in 20:5(n-3) and 22:6(n-3) with increased marine oil intake. The ratio 20:3(n-6)/20:4(n-6) correlated significantly with 20:5(n-3), but not with 22:6(n-3), in all tissues except the heart, suggesting an inhibitory effect of 20:5(n-3) on delta-5-desaturase enzyme.