Response of the parotid gland of the brushtail possum, Trichosurus vulpecula, to adrenergic stimulation

Abstract

Adrenergic stimulation of parotid secretion was investigated in anaesthetised brushtail possums to ascertain fluid secretion rates and salivary composition. Because neither α- nor β-adrenergic stimulation evoked saliva output, infusion of the adrenergic agonists was superimposed on a pre-existing bethanechol-stimulated flow. Isoprenaline infusion (2.4 nmol min −1) increased salivary amylase activity, [protein]; [HCO 3]; [PO 4] and [Ca], and amylase/Ca and protein/Ca ratios; reduced [Cl]; [K] and osmolality; but did not alter H + activity; [urea]; [Na]; [Mg]; amylase/protein or saliva/plasma urea ratios. These data are consistent with isoprenaline stimulating acinar secretion of protein, Ca and PO 4 but not the ion transport necessary for primary fluid formation at the endpieces and modifying transport of monovalent ions in the excurrent ducts. Consequently, the possum parotid has β-adrenergic receptors in both the endpieces and excurrent ducts. Phenylephrine infusions at 2.4 and 24 nmol min −1 were without effect whereas phenylephrine at 240 nmol min −1 caused changes in salivary composition which paralleled those for isoprenaline administration but were generally of lesser magnitude. Thus, the possum parotid has few or no α-adrenergic receptors and the salivary response elicited was the result of cross-reaction of phenylephrine with β-adrenergic receptors.