RESPONSE OF SOME LEGUMINOUS PLANTS TO SPECIFIC RHIZOBIAL INOCULATION TIMING WITHIN GERMINATION STAGE

Abstract

The present work was conducted to: a) Evaluation the effect of some abiotic stress tolerance of the specific rhizobial strains and its phenotypic characterization. b) Evaluation the effect of the timing application of the specific rhizobial liquid inoculationto some leguminous plants on the nodulation process. c) Evaluation the response of some hybrids of the same leguminous plants to the timing application of its specific rhizobial liquid inoculation., and d) Reflection between the response of some leguminous plants and their hybrids to the timing application of its specific rhizobial liquid inoculation on the plant growth and crop quality. To select the most three efficient strains to employed in the field experiment. Some specific rhizobial strains were subjected to some abiotic stress tolerance and its phenotypic characterization. Antibiotics, temperature, salt (NaCl) lead (Pb) and sodium azide tolerance, IAA, exopolysaccharides (EPS), were determined, as well as morpho-cultural characterization (Gram staining, colony size, shape, border, elevation, color, mucosity, transparency, scanning electron micro graph of the same tested specific rhizobial strains, were performed. Three field experiments were conducted at the same site at Om Saber Village-Tahrir regions - Beheira Governorate, Egypt (Latitude 30o.40´N- Longitude 30o.33´E) (sandy soils presented newly reclaimed soil). The first, second and third experiments were carried out on soybean (Glycine max), common beanplants (Phaseolus vulgarisL.) and peanut (Arachis hypogaea L.), respectively. Each experiment was carried using two cultivars of the cultivated leguminous plants. These treatment of each experiments were complete randomized block plot design. The planting dates were at 15 April and 15 Mai, 1st September 2019 and for Soybean, common and peanut bean plants, respectively.