Response of Soil Bacterial Community Diversity and Composition to Time, Fertilization, and Plant Species in a Sub-Boreal Climate.

Honghong Li,Frederick L Stoddard,Kristina Lindström,Anu Mikkonen,Petri Penttinen

doi:10.3389/fmicb.2020.01780

Abstract

Pastures are an important part of crop and food systems in cold climates. Understanding how fertilization and plant species affect soil bacterial community diversity and composition is the key for understanding the role of soil bacteria in sustainable agriculture. To study the response of soil bacteria to different fertilization and cropping managements, a 3-year (2013–2015) field study was established. In the split-plot design, fertilizer treatment (unfertilized control, organic fertilizer, and synthetic fertilizer) was the main plot factor, and plant treatment [clear fallow, red clover (Trifolium pratense), timothy (Phleum pratense), and a mixture of red clover and timothy] was the sub-plot factor. Soil bacterial community diversity and composition, soil properties, and crop growth were investigated through two growing seasons in 2014 and 2015, with different nitrogen input levels. The community diversity measures (richness, Shannon diversity, and Shannon evenness) and composition changed over time (P < 0.05) and at different time scales. The community diversity was lower in 2014 than in 2015. The temporal differences were greater than the differences between treatments. The overall correlations of Shannon diversity to soil pH, NO, NH, and surplus nitrogen were positive and that of bacterial richness to crop dry matter yield was negative (P < 0.05). The major differences in diversity and community composition were found between fallow and planted treatments and between organic and synthetic fertilizer treatments. The differences between the planted plots were restricted to individual operational taxonomic units (OTUs). Soil moisture, total carbon content, and total nitrogen content correlated consistently with the community composition (P < 0.05). Compared to the unfertilized control, the nitrogen fertilizer loading enhanced the temporal change of community composition in pure timothy and in the mixture more than that in red clover, which further emphasizes the complexity of interactions between fertilization and cropping treatments on soil bacteria.

Highlights

Grassy pastures are an important part of agricultural systems where ruminants are the main way for producing food under cold, dry, or other stressful conditions
We have previously shown that the management and the season induced temporal changes in crop growth and soil properties, with the legume–grass mixture providing particular advantages over the pure stands of red clover (Trifolium pratense) and timothy (Phleum pretense) in terms of improved pasture yield and reduced potential N loss (Li et al, 2019)
The community diversity and the composition changed at different time scales, with the diversity changing between years, whereas the composition changed between seasons within a year

Summary

Introduction

Grassy pastures are an important part of agricultural systems where ruminants are the main way for producing food under cold, dry, or other stressful conditions. To sustain or improve pasture production, farming systems have been widely intensified through nitrogen (N) fertilizer inputs (Tilman et al, 2002; Stott and Gourley, 2016). Soil bacteria are critical for N flows through biological nitrogen fixation (BNF) that provides N for crop growth, nitrification by oxidizing NH+4 into NO−3 , denitrification by reducing NO−3 into NO, N2O, and N2, and in dissimilatory NO−3 reduction to ammonium (Philippot et al, 2007; Franche et al, 2009; Jia and Conrad, 2009; Yoshida et al, 2009; Robertson and Groffman, 2015)

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Results

Discussion

Conclusion