Abstract
METHODS: BD-MSCswereobtained from theperipheral bloodof 810week-oldCD1wild type or FVB-Tg(CAG-luc,-GFP)L2G85Chco/ J mice, which express firefly luciferase and cytoplasmic eGFP constitutively in all cells, by a novel method utilizingOptiPrep density gradient isolation and hepatocyte stimulation. Cells were characterized by fluorescence-activated cell sorting (FACS). For calvarial defect healing, cells (5.0x105) were seeded onto hydroxyapatite-poly(lactic-co-glycolic acid) (HA-PLGA) scaffolds and placed onto critical-sized (4 mm) calvarial defects on CD1 athymic nude mice. For wound healing, BDMSCs were seeded onto pullulan-collagen composite dermal hydrogels, and transplanted (2.5 105 cells per wound) onto 6 mm splinted full thickness excisional wounds on the dorsum of FVB/NJ mice.
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