Response of Sesbania sesban (L.) Merr. to rhizobial inoculation in an N-deficient soil containing low numbers of effective indigenous rhizobia

Abstract

Sesbania sesban (L.) Merr. (sesbania) is a fast growing N2-fixing widely used as an improved fallow species by smallholder farmers in eastern and southern Africa to restore fertility of their N-deficient soils. In order to establish the need for inoculation, the population of sesbania rhizobia in soil collected from a site where the species is intended for introduction was assessed using the most probable number (MPN) plant infection assay. The MPN of sesbania rhizobia was low (21, 6–81 fiducial limits at P=0.05 g−1soil) but with N2-fixation potential comparable to sesbania inoculant strain KFR 651. Evaluation of an indigenous sesbania rhizobial isolate GSS 1 from the MPN assay in potted field soil showed that it was more effective than strain KFR 651 in terms of plant growth and shoot dry matter (DM) accumulation at 9 and 12 weeks after planting, respectively. Total shoot N content was also higher for plants inoculated with isolate GSS 1 than inoculant strain KFR 651 and uninoculated control treatments 12 weeks after planting. These results demonstrate that it is better to inoculate with effective indigenous than exogenous rhizobia where the need for inoculation has been established.