Response of rice genotypes to zinc fertilizer detected using RAPD

Abstract

Rice nutrition as a staple food in Asia is important for human metabolism and health. One of the global warming effects is micro element reduction in rice, including Zn. Fertilizer application of Zn(SO4).2H20 was studied to investigate effect of Zn on some genotypes of local rice (white rice cv. Mansur, red rice cv. Cendana, sticky rice and black sticky rice) from Jatiluwih, Tabanan, Bali using RAPD marker. Experiments was conducted on plastic pot containing 2 kg soil enriched with Zn(SO4).2H2O with concentration as followed 0.0; 2.5; 5,0 mg/kg soil. Each treatment consists of three replicates with 2 sub unit for each replicates. There were two seedlings per pot. Pots were placed in open field at Denpasar, Bali. Rice seedlings were grown until 8 weeks after planted. Seedlings were kept waterlogged during 8 weeks growth period. Random Amplified Polymorphic DNA (RAPD) marker was employed to detect polymorphism between treatments. Primers used were OPB12 and OPH1. RAPD showed that each genotype had different response to Zn treatment.