Response of prokaryotic and eukaryotic community structure to inorganic and organic nutrient additions in a eutrophic coastal ecosystem

Abstract

Event Abstract Back to Event Response of prokaryotic and eukaryotic community structure to inorganic and organic nutrient additions in a eutrophic coastal ecosystem Marta Hernández-Ruiz1*, Esther Barber-Lluch1, Xosé A. Álvarez-Salgado2, Mar Nieto-Cid2, Francisco G. Figueiras2, Belén Arbones2, Isabel G. Teixeira2, Cristina Sobrino1, Emilio Fernández1 and Eva Teira1 1 University of Vigo, Departamente de Biología y Ecología Animal, Spain 2 IIM-CSIC, Instituto de Investigaciones Marinas, Spain Coastal oceans are known to receive increasing inputs of reactive N as a result of the anthropogenic alteration of N cycling at the global scale, which may greatly alter nutrient supply to microbial plankton communities. The influence of nutrient availability (mainly inorganic N and P and/or organic C) on species composition has been widely reported for both eukaryotes and prokaryotes. The Ría de Vigo is a eutrophic embayment located in the coastal system of the NW Iberian Peninsula. Previous studies in the area revealed a highly variable response of microbial plankton to nutrient additions, a strong stimulation of phytoplankton growth after inorganic (N and P) and organic (C and N) nutrient addition and a strong carbon limitation of bacterial activity. To gain understanding on the effect of nutrient inputs on the functioning of this coastal ecosystem, the short-term effects of inorganic N and P (nitrate, ammonium, phosphate) and organic C and N (glucose, amino acids) inputs, added separately as well as jointly, on eukaryotic and prokaryotic community composition were studied in microcosm experiments conducted under contrasting hydrographic conditions. Changes in microbial diversity were analyzed by ARISA (automatic rRNA intergenic spacer analysis) and two sets of primers specific for bacteria and eukaryotes. The results obtained in these experiments showed that changes in microbial communities were clearly determined by the initial microbial assemblage. Changes in prokaryotic and eukaryotic community composition were closely related to changes in metabolic rates. In contrast to previous observations, prominent changes in bacterial community composition occurred only after inorganic and organic nutrient additions, which suggest a C and P co-limitation of bacteria during the sampling periods. Major eukaryotic community composition changes were observed in response to inorganic additions. Keywords: eukaryotic diversity, prokaryotic diversity, ARISA, Nutrient additions, Microcosm Experiment Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BIODIVERSITY, CONSERVATION AND COASTAL MANAGEMENT Citation: Hernández-Ruiz M, Barber-Lluch E, Álvarez-Salgado XA, Nieto-Cid M, Figueiras F, Arbones B, Teixeira I, Sobrino C, Fernández E and Teira E (2014). Response of prokaryotic and eukaryotic community structure to inorganic and organic nutrient additions in a eutrophic coastal ecosystem. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00094 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Apr 2014; Published Online: 18 Jul 2014. * Correspondence: Miss. Marta Hernández-Ruiz, University of Vigo, Departamente de Biología y Ecología Animal, Vigo, Pontevedra, 36200, Spain, marta.hdezr@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marta Hernández-Ruiz Esther Barber-Lluch Xosé A Álvarez-Salgado Mar Nieto-Cid Francisco G. Figueiras Belén Arbones Isabel G. Teixeira Cristina Sobrino Emilio Fernández Eva Teira Google Marta Hernández-Ruiz Esther Barber-Lluch Xosé A Álvarez-Salgado Mar Nieto-Cid Francisco G. Figueiras Belén Arbones Isabel G. Teixeira Cristina Sobrino Emilio Fernández Eva Teira Google Scholar Marta Hernández-Ruiz Esther Barber-Lluch Xosé A Álvarez-Salgado Mar Nieto-Cid Francisco G. Figueiras Belén Arbones Isabel G. Teixeira Cristina Sobrino Emilio Fernández Eva Teira PubMed Marta Hernández-Ruiz Esther Barber-Lluch Xosé A Álvarez-Salgado Mar Nieto-Cid Francisco G. Figueiras Belén Arbones Isabel G. Teixeira Cristina Sobrino Emilio Fernández Eva Teira Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.