Response of porcine epithelial rests of Malassez to stimulation by interleukin-6.

Abstract

To investigate the proliferation and migration of epithelial cell rests of Malassez (ERM) after stimulation with IL-6. Porcine-derived ERM were seeded on Dulbecco's modified Eagle's Medium, and IL-6 (100pgmL-1 ) was incorporated into the culture medium. The WST-1 assay was performed to evaluate cell proliferation, and absorption was measured at 450nm. A wound-healing assay and immunofluorescence assay for integrin α3 were conducted to investigate migration. The Kruskal-Wallis test and the Mann-Whitney U-test with Bonferroni correction were used to analyse data of WST-1 and wound-healing assays. Cell proliferation following the stimulation by IL-6 increased over time, with a significant increase being observed at 6h (P<0.05), but not in a concentration-dependent manner. Cell proliferation was significantly greater in IL-6-treated ERM than in nontreated ERM (P<0.05). The results of the wound-healing assay revealed earlier closure in IL-6-treated ERM (P<0.05). In the immunofluorescence assay, integrin α3 was detected at the edge of cell processes adjacent to the wound area. A neutralized antibody abrogated the effects of the IL-6 stimulation in cell proliferation and migration. IL-6 promoted the proliferation and migration of porcine ERM invitro.