Response of photosynthesis in the leaves of cucumber seedlings to light intensity and CO2 concentration under nitrate stress

Abstract

The effects of 2 nitrate levels, 14 (CK) and 140 mmol L^{-1} (T), on the leaf gas exchange variables of cucumber (Cucumis sativus L. cv. Xintaimici) seedlings grown in hydroponic culture were investigated. Photosynthetic light- and CO_{2}-response curves from CK and T seedlings were determined and used for the analysis of photosynthetic capacity. The results showed that nitrate stress resulted in a significant reduction of net photosynthesis of T seedlings compared with CK. At the same time, the apparent quantum yield, light-saturated net photosynthesis, carboxylation efficiency, and CO_{2}-saturated net photosynthesis in nitrate-stressed cucumbers also decreased significantly with the increase of treatment time. At 12 days, the apparent quantum yield, light-saturated net photosynthesis, carboxylation efficiency, and CO_{2}-saturated net photosynthesis in nitrate-stressed cucumbers were 47%, 60%, 64%, and 54% lower than CK, respectively. The relative effect of stomatal resistance on photosynthesis (S%) in nitrate-stressed cucumber seedlings increased significantly during the first 8 days and returned to the level of CK thereafter. This evidence indicates that not only stomatal but also nonstomatal limitations might be involved in the reduction of net photosynthetic rate in nitrate-stressed seedlings.