Abstract
Parkia biglobosa, a leguminous savanna species, is a threatened species in some West African countries especially Nigeria, due to deforestation and unsustainable exploitation. To conserve this economically important genetic resource, attempt on its micropropagation was carried out using nodal cuttings of the young stem as the explants. The explants were thoroughly surface sterilized, disinfected and cultured in Murashige and Skoog (MS) basal medium. About three weeks after inoculation, 95% sprouting was recorded. The new spouts were subcultured into MS medium fortified with varied concentrations (0.00, 0.25, 0.50, 0.75, and 1.00 mg/L) of benzylaminopurine (BAP) and indole-3-butyric acid (IBA) and then transferred to the growth chamber at 27±1oC. The 0.75 mg/L of BAP produced highest shoot elongation of 3.60 cm while 1.0 mg/L caused shrinkage of the explants in relation to the control (0.00 mg/L concentration). Attempt to root the plantlets with the above-stated concentrations of IBA led to callogenesis and the callus mass increased with the increasing concentration of IBA.Keywords: callus induction, explant, micropropagation, Parkia biglobosa
Highlights
Parkia biglobosa, is an economically important leguminous species that is indigenous to Africa from where it spread to other regions
The part of plant used as explants for the micropropagation has effect on shoot elongation
The apices and axillary node explants cultured on Murashige and Skoog (MS) basal medium only without any hormone showed that elongation occurred faster with shoot apices (Plate 1)
Summary
Parkia biglobosa (locust bean tree), is an economically important leguminous species that is indigenous to Africa from where it spread to other regions. The tree is widely distributed on sandy loam soil of Sudan and Guinea savanna areas of Nigeria (Aliero et al, 2001). P. biglobosa is a multipurpose fruit tree species with wide range of economic values. Its chief products are the seeds extracted from pods for condiments in stews and soups, the fruit pulp is used for soil amendment in agriculture (Booth and Wickens, 1988). Both the tree and the fruit has wide range of utilization in pharmacopeia and traditional medicine. P. biglobosa is used in agroforestry practice across the tropics (Nair, 1993), soil amelioration or improvement, shelter belt and to mitigate global warming (Tomilinson et al, 1995)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
