Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

Abstract

In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.