Abstract
Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine.
Highlights
Amnion is a thin membrane surrounding the embryo/fetus, without a supply of blood vessels
For preliminary characterization of the content of biologically active factors secreted by the amnion into culture medium we used the peptide micro array technique with panel of antibodies directed against human growths factors representing major families: epidermal and fibroblast growth factors (EGF, FGF), hepatocyte growth factor (HGF), vasculogenic and angiogenic growth factors (VEGF, PLGF, AR), neurotrophic factors (NGF, GDNF, NT), hematopoietic growth factors (G-CSF, granulocyte–macrophage colony– stimulating factor (GM-CSF), M-SCF, SCF), insulin-like growth factors (IGF, IGFBP), platelet-derived growth factors (PDGF), and transforming growth factors (TGF)
Having established that numerous growth factors are present in hAM CCM we investigated its effect on migration and proliferation of human normal (HUVECs, bone marrow mononuclear cells (BM MNCs)) and leukemia cells
Summary
Amnion is a thin membrane surrounding the embryo/fetus, without a supply of blood vessels. Two populations of cells can be distinguished within the human amnion: epithelial cells and mesenchymal stromal/stem cells. The amniotic membrane (AM) functions are angiogenic, immunogenic, and anti-inflammatory [1]. Amniotic membrane is a convenient source of cells producing potentially therapeutic factors [2].
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