Response of gut microbiota and immune function to hypoosmotic stress in the yellowfin seabream (Acanthopagrus latus)

Abstract

Osmotic stress is associated with heightened immune functions and altered microbiota in the fish intestine. In this study, we explored the effects of hypoosmotic stress on the intestine of euryhaline yellowfin seabream (Acanthopagrus latus) after acute exposure to brackish water, low-saline water, and freshwater environments. The results showed that hypoosmotic stress reshaped the composition of the microbial community and altered the gene expression in the intestine. Probiotics Lactobacillus and Pseudomonas showed higher relative abundance in a brackish water environment, whereas pathogenic bacteria, including Vibrio and Aeromonas, were more abundant in the freshwater environment. At the transcriptional level, osmoregulation-related genes were identified as up/down regulated differentially expressed genes (DEGs) as well as a series of immune-related DEGs associated with pathogen recognition, antimicrobial ability, pro-inflammatory cytokines, cell apoptosis, and antioxidant defense. Physiological analysis showed that Na+ K+-ATPase activity was significantly inhibited by hypoosmotic stress in freshwater. Meanwhile, the intestinal antioxidant defense system of yellowfin seabream was challenged. Correlation network analysis demonstrated the close interactions among intestinal microbes, differentially expressed genes, and physiological parameters. This study provides the critical insights into the function of the intestine fish encountering hypoosmotic stress.