Abstract
Spheroplasts of Escherichia coli , produced by lysozyme-EDTA treatment of the argr − strain 977 (derived from strain K12), synthesize enzymes of the arginine system at derepressed rates, in an enriched arginine-sorbitol medium at 37° with aeration. Addition of extracts from arginine-grown (repressed) cells of the argr + strain 961 (also derived from K12), but not of extracts from (derepressed) cells of 977, brings about a pronounced, concentration-dependent repression of the formation of arginine enzymes, including acetylornithine δ-transaminase, acetylornithinase, and ornithine transcarbamylase.
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