Abstract
Abstract Aflatoxin B1 inhibits cell cleavage in fertilized mollusk eggs without preventing fertilization or nuclear division. The reaction is very dramatic and positive since fertilized eggs in the presence of aflatoxin are multinuclear, whereas the controls have multiplied to multicellular larvae. The bioassay requires a minimum of technique and training and is sensitive to concentrations of 0.05 μg/ml. Because the reaction is observed with the aid of a low-powered microscope (70X), the concentration of aflatoxin required can be reduced considerably below 0.05 μg. The test required two to four hours for completion when observing egg division and 18 hours for swimming larvae.
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