Response of Douglas-fir and amabilis fir to split-root nutrition with inorganic and organic nitrogen

Abstract

There is limited understanding of the spatial plasticity of conifer root growth in response to inorganic and organic nitrogen (N). In this study, slow-growing amabilis fir and fast-growing Douglas-fir, and slow- and fast-growing seedlots of the latter species were examined for their ability to proliferate roots preferentially in compartments of sand/peat medium enriched in organic and inorganic forms of N. In one experiment, N was supplied as 7.1 or 0.71 mM ammonium, nitrate and ammonium nitrate, and in a second experiment, N was supplied as ammonium or glycine. The seedlings’ ability to compensate for the starvation of a portion of the root system was assessed by measuring biomass of leaves, stems and roots, and foliar N concentration. Both fast- and slow-growing seedlots of Douglas-fir and slow-growing amabilis fir were able to proliferate roots in compartments of soil enriched with inorganic and organic N. In the first experiment, whole plant and root biomass was greatest when N was provided as ammonium followed by nitrate, and in the second experiment, seedling whole and root biomasses did not differ between ammonium and glycine treatments. All seedlings were able to compensate for the starvation of a portion of the root system, thus total plant biomass did not differ between split-root treatments; however, foliar N contents were lower in the 7.1/0.71 mM inorganic N split-root treatments. Foliar N concentrations were also lower in seedlings supplied with glycine.