Abstract
The characteristics underlying the response of cytosolic Ca 2+ ([Ca 2++] i ) to hypercapnic acidosis in clusters of cultured glomus cells of the adult rabbit carotid body were evaluated using fura-2 microscopic fluorometry. Application of CO 2 by bubbling through both superfusions of bicarbonate-buffered saline and HEPES-buffered saline produced a rapid and sustained increase in [Ca 2+] i. The [Ca 2+] i response increased correspondingly with a rise in concentrations of CO 2 to 20% but at a point between 20 and 50% adapted to CO 2 and it decreased its linear assent. The hypercapnic acidosis-induced increased of [Ca 2+] i was diminished by removing external Ca 2+. Also the [Ca 2] i response was reduced in dose-dependent fashion by the addition of the voltage-gated Ca 2+ channel blocker D600. An additional response of [Ca 2+] i to acetate was also diminished by Ca 2+ removal. These results suggest that the [Ca 2+] i response to hypercapnic acidosis involves an influx of external Ca 2+ through voltage-gated Ca 2+ channels.
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