Response of Castor Seedling Roots to Combined Pollution of Cd and Zn in Soils

Abstract

Castors are used to remediate heavy-metal-polluted soils due to their good ability to accumulate heavy metals. However, only limited studies addressed the interaction between heavy metals and castor seedling roots. In this study, the physiological response of castor seedling roots to Cd and Zn stress, and the change in trace elements in rhizosphere and non-rhizosphere soils were investigated. The results showed that, with an increase in Cd concentration, the accumulation of Zn in roots decreased by 20%, indicating a competition between Cd and Zn accumulation. The increase in Cd content enlarged the difference in nutrient contents at different depths: the amounts of P, Fe, and Mn were more in rhizosphere soils than in non-rhizosphere soils, while the amount of K showed an opposite trend. The addition of Cd and Zn stimulated root growth, but root activity was reduced. The addition of Cd and Zn affected the root cell morphology, including increases in the root cortex thickness and the root xylem area. The contents of the enzymes SOD, POD, and MDA increased with the addition of Cd and Zn, while the activity of CAT first increased and then decreased. There was no significant change in the soluble protein content. The decrease in IAA oxidase content, from 40.1% to 72.7%, was conducive to plant growth. To sum up, high contents of Cd and Zn in soils not only affect the root morphology and increase the gap in the contents of K, P, Fe, and Mn between rhizosphere and non-rhizosphere soils but also change the SOD, POD, MDA, and IAA contents in the root, so as to reduce the amount of root damage caused by the external environment.