Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure.

Yun Wang,Jun Wang,Heizhao Lin,Lihui Liu,Mingjun Fu,Yafei Duan,Jianhua Huang

doi:10.1186/s40064-016-2381-4

Yun Wang, Jun Wang + Show 5 more

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https://doi.org/10.1186/s40064-016-2381-4

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Journal: SpringerPlus	Publication Date: Jun 22, 2016
Citations: 12	License type: CC BY 4.0

Affiliation: Chinese Academy of Fishery Sciences

Abstract

Glutathione S-transferases (GSTs) are a family of multifunctional phase II enzymes that are involved in the detoxification of exogenous and endogenous compounds. In this study, a full-length cDNA of Mu-class GST (PmMuGST) was isolated from the hepatopancreas of Penaeus monodon using rapid amplification of cDNA ends method. The full length cDNA of PmMuGST is 867 bp, contains an open read frame of 660 bp, and encodes a polypeptide of 219 amino acids with a molecular mass of 25.61 kDa and pI of 6.15. Sequence analysis indicated that the predicted protein sequence of PmMuGST was very similar to (86 %) that of Litopenaeus vannamei. A conserved domain of GST_N_Mu_like (PSSM: cd03075) and GST_C_family_superfamily_like (PSSM: cl02776) was indentified in PmMuGST. Real time quantitative RT-PCR analysis indicated that PmMuGST was present in all of the tested tissues. PmMuGST transcripts both in the hepatopancreas and in the muscle were significantly induced after 14 days of treatment with a low dosage of AFB1 (50 μg/kg) exposure and were significantly inhibited after 42 and 56 days of a high dosage of AFB1 (1000, 2500 μg/kg AFB1) exposure. Taken together, the Mu-class GST from P. monodon was inducible and was involved in the response to AFB1 exposure.

Highlights

To counteract the harmful effects of endogenous and exogenous compounds, such as drugs and mycotoxins, living organisms employ an array of enzymatic detoxification processes that have been classified into three distinct phases (Bathige et al 2014)
Growth performance and survival of P. monodon Shrimp fed with diets containing AFB1 (50–100 μg/kg) had lower WG as than diets containing 0 (D0) group, but there were no significant differences (P > 0.05)
Shrimp fed with diets containing AFB1 (500–2500 μg/kg) exhibited significantly lower WG compared with the control group (P < 0.05)

Summary

Introduction

To counteract the harmful effects of endogenous and exogenous compounds, such as drugs and mycotoxins, living organisms employ an array of enzymatic detoxification processes that have been classified into three distinct phases (Bathige et al 2014). Wang et al SpringerPlus (2016) 5:825 many classes of GSTs have been cloned and characterized in several aquatic species including the Pacific oyster Crassostrea gigas (Boutet et al 2004), rock shell Thais clavigera (Rhee et al 2008), disk abalone Haliotis discus discus (Wan et al 2008a), yellow catfish Pelteobagrus fulvidraco (Ku et al 2014) and Manila clam Ruditapes philippinarum (Bathige et al 2014). Two classes of GSTs (FcMuGST, FcThetaGST) were cloned from Chinese shrimp Fenneropenaeus chinensis, and the FcMuGST transcript was determined to be increased in response to Vibrio anguillarum infection, while FcThetaGST showed little change at the transcript level. GSTs play vital roles in the detoxification and immune system of aquatic animals, and many studies have undertaken biochemical characterization and transcription profiles of GSTs after pathogenic and toxic chemical exposures. There is minimal information regarding the molecular structure of GSTs and their response to AFB1 from the black tiger shrimp

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