Abstract
Antidiuretic hormone (ADH) increased the adenosine 3′,5′-cyclic monophosphate (cAMP) content of cultured cells of the MDCK line, derived from normal dog kidney, after activation of adenylate cyclase. Parathyroid hormone and insulin did not affect the cAMP content of the cells and ADH did not increase the cAMP content of HeLa or 3T6 cells. Thus the effect of ADH on MDCK cells was specific. ADH activated the protein kinase of MDCK cells. Conversion of cAMP-dependent protein kinase to a cAMP-independent catalytic subunit in the ADH-treated cells was demonstrated by glycerol density gradient centrifugation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
