Abstract
The valuable medicine Shiraia bambusicola P. Henn. and its major active substance hypocrellin exert unique curative effects on skin diseases, diabetes, and cancers. The wild S. bambusicola is endangered due to its harsh breeding conditions and long growth cycle. It is one of the effective ways to utilize the resources sustainably to produce hypocrellin by fermentation of S. bambusicola. PB90 is a protein elicitor isolated from Phytophthora boehmeriae to induce the useful metabolites production in fungi. In this work, PB90 was selected to promote the synthesis hypocrellin by S. bambusicola. To evaluate the effect of PB90 on S. bambusicola, it was found that the induced cells showed decreased biomass, increased cell wall permeability, rapid induction of secondary metabolites, and significant increase of some enzyme activities, which confirmed a strong activation of phenylalanine/flavonoid pathways. Studies on signal molecules and gene expression level in S. bambusicola treated with PB90 have found that hydrogen peroxide (H2O2) and nitric oxide (NO) are necessary signal molecules involved in the synthesis of hypocrellin in elicited cells, and increased their signal levels through mutual reaction. We have showed for the first time, the response mechanism of hypocrellin biosynthesis from S. bambusicola to PB90, which may be not only establish a theoretical foundation for the application of PB90 to the mass production of S. bambusicola, but can also motivate further research on the application of PB90 to the conservation and sustainable utilization of other medical fungi.
Highlights
Secondary metabolite synthesis is strictly controlled in fungus growing under normal conditions (Xu et al 2011)
Few researchers have studied the effect of PB90 on fungal metabolites, and no one has studied the effect of PB90 on the secondary metabolites of S. bambusicola
That the production of hypocrellin was significantly increased in S. bambusicola cells treated with PB90, and the yield was up to 278.71 mg/L
Summary
Many problems in hypocrellin biosynthesis are still encountered, for example, optimize contents are repeated (Du et al 2013; Lei et al 2017; Sun et al 2017), the hypocrellin synthesis mechanism is unknown (Gao et al 2018), the status of strains used is unclear, and the active metabolite content is low (Du et al 2013; Sun et al 2017) This series of problems hinders the commercial production of hypocrellin and can be solved by exploring ways to improve hypocrellin yield
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
